User Guide Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product User Guide](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-User-Guide.jpg)
Perform in Clean Area
1) Plate the Master Mix
a. Load the Co-Dx Box software.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Load the Co-Dx Box software](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Load-the-Co-Dx-Box-software.jpg)
b. In the Clean area, clean work surfaces with water > 10% bleach > water > 70% alcohol (in that order), or another equivalent method of cleaning that disinfects and degrades nucleic acids.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - In the Clean area, clean work surfaces](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-In-the-Clean-area-clean-work-surfaces.jpg)
c. Thaw the master mix (MM) and the nuclease-free water (NFW) (for the no-template control [NC]), on ice.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Thaw the master mix (MM)](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Thaw-the-master-mix-MM.jpg)
d. Briefly vortex and centrifuge the MM and NFW reagents.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Briefly vortex and centrifuge the MM](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Briefly-vortex-and-centrifuge-the-MM.jpg)
e. Add 5 µL of MM into every well that will be used (1 well for NC, 1 well for positive control [PC], and 1 well for each sample).
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Add 5 µL of MM into every well that will be used](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Add-5-µL-of-MM-into-every-well-that-will-be-used.jpg)
f. Pipet 5 µL of NFW into only the NC well and cap the NC well.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Pipet 5 µL of NFW into only the NC well and cap the NC well](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Pipet-5-µL-of-NFW-into-only-the-NC-well-and-cap-the-NC-well.jpg)
g. Place reagents back into the freezer.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Place reagents back into the freezer](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Place-reagents-back-into-the-freezer.jpg)
Perform in Positive Area
2 Plate Samples and Positive Control (PC)
a. Load the Co-Dx Box software.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Load the Co-Dx Box software 2](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Load-the-Co-Dx-Box-software-2.jpg)
b. In the Positive area, clean work surfaces with water > 10% bleach > water > and 70% alcohol (in that order).
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - In the Positive area, clean work surfaces with water](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-In-the-Positive-area-clean-work-surfaces-with-water.jpg)
C. Thaw the PC on ice.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Thaw the PC on ice.](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Thaw-the-PC-on-ice..jpg)
d. Briefly vortex and centrifuge all reagents.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Briefly vortex and centrifuge all reagents.](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Briefly-vortex-and-centrifuge-all-reagents..jpg)
e. Perform sample extraction, either using RNA extraction or lysis.
f. Add 5 µL of the sample extract to the appropriate wells and cap the sample extract wells.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Add 5 µL of the sample extract to the appropriate](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Add-5-µL-of-the-sample-extract-to-the-appropriate.jpg)
g. Add 5 µL PC to the PC well and cap the PC well.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Add 5 µL PC to the PC well and cap the PC well](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Add-5-µL-PC-to-the-PC-well-and-cap-the-PC-well.jpg)
h. Place capped tubes in the thermocycler, in appropriate locations and fill the rest of the carousel with blank tubes.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Place capped tubes in the thermocycler](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Place-capped-tubes-in-the-thermocycler.jpg)
i. (Optional): Cap all tubes and place samples and reagents back into the freezer.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - (Optional) Cap all tubes and place sam- ples and reagents back into the freezer](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Optional-Cap-all-tubes-and-place-sam-ples-and-reagents-back-into-the-freezer.jpg)
Perform in Co-Dx Box Software
3 Start the Run
a. In the Co-Dx Box software, click New.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product -In the Co-Dx Box software, click New](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-In-the-Co-Dx-Box-software-click-New.jpg)
b. Select an assay template and click OK.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Select an assay template and click OK.](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Select-an-assay-template-and-click-OK..jpg)
c. Under Run Setup, select Samples and type sample information in the appropriate order.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Under Run Setup, select Samples and type sample information in the appropriate order](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Under-Run-Setup-select-Samples-and-type-sample-information-in-the-appropriate-order.jpg)
d. Click Information and in the Notes field, type any needed information.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Click Information and in the Notes field, type any needed information](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Click-Information-and-in-the-Notes-field-type-any-needed-information.jpg)
e. Select the appropriate thermocycler and from the drop-down select Start Run.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Select the appropriate thermocycler and from the drop-down select Start Run.](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Select-the-appropriate-thermocycler-and-from-the-drop-down-select-Start-Run..jpg)
f. Verify the tube clamp is in place and the appropriate reaction volume is listed, and select Start.
![Co-Dx ZDC-R-001 Logix Smart Zika Dengue and Chikungunya RUO Product - Verify the tube clamp is in place and the appropriate reaction volume is listed, and select Start](http://manual.directory/wp-content/uploads/2022/12/Co-Dx-ZDC-R-001-Logix-Smart-Zika-Dengue-and-Chikungunya-RUO-Product-Verify-the-tube-clamp-is-in-place-and-the-appropriate-reaction-volume-is-listed-and-select-Start.jpg)
The run initializes and a progress bar gives an estimated time to completion.